Expression & Purification

(1) Gene Design and Expression System Optimization:

• Membrane protein sequence analysis and codon optimization
• Expression vector construction
• Tag design and fusion protein strategy optimization
• Expression system screening

Support for various expression systems:

• Mammalian cell expression systems
• Insect cell expression systems
• Escherichia coli expression systems
• Other customized expression systems

(2) Membrane Protein Expression Condition Optimization

Systematic optimization addressing key issues in membrane protein expression:

• Expression host screening
• Culture condition optimization
• Expression level enhancement
• Protein stability assessment

(3) Membrane Protein Purification and Quality Control:

• Membrane protein extraction
• Detergent screening and optimization
• Affinity purification
• Size-exclusion chromatography (SEC)
• Protein purity analysis
• Monodispersity assessment

Delivery of high-quality membrane protein samples suitable for:

• Functional assays
• Structural studies
• Drug screening

Project timelines vary depending on the target protein type, expression system, and optimization complexity:

Note: For complex membrane proteins (such as certain GPCRs, ion channels, and transporters), the actual project timeline requires dynamic adjustment based on project progress, given the significant variations in expression levels, stability, and purification conditions.

• Gene design plan;
• Records of expression condition optimization;
• Expression detection data;
• SDS-PAGE analysis results;
• Western Blot results (if applicable);
• SEC purification profile;
• Protein purity analysis results;
• Protein concentration and batch information;
• Protein stability evaluation data.
• Purified membrane protein sample;
• Instructions for protein sample storage conditions;